STN EN ISO 20418-1
| Name: | STN EN ISO 20418-1 |
| Validity: | Valid |
| Number of pages: | 22 |
| Language: |
EN
|
| Paper: | 14,10€ |
| Electronic version |
a) Only read (without ability to print and copy)
12,69€ b) Without ability to print, with ability to copy (printscreen) 14,10€ c) With ability to print and copy (printscreen) 18,33€ |
| Slovak title | Textílie. Kvalitatívna a kvantitatívna proteomická analýza niektorých vlákien zo zvieracích chlpov. Časť 1: Detekcia peptidov použitím LC-ESI-MS s redukciou proteínov |
| English title | Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 1: Peptide detection using LC-ESI-MS with protein reduction (ISO 20418-1:2018) |
| Release Date: | 01. 11. 2018 |
| Date of withdrawal: | |
| ICS: | 59.060.01 |
| Sorting character/National clasification code | 80 8931 |
| Level of incorporation: | idt ISO 20418-1:2018, idt EN ISO 20418-1:2018 |
| Official Journal | 10/18 |
| Amendments | |
| Replaced by: | |
| Repleces: | |
| Note in Official Journal: | |
| Subject of the standard: | This document specifies a qualitative and quantitative testing method to determine the content of wool, cashmere, yak fibres and their blends in textiles by microscope preliminary screening, protein extraction, enzymatic digestion and specific peptides detection using a liquid chromatography-mass spectrometer equipped with electrospray ionization source (LCI-ESI-MC). This method can be applied to relevant textile products at each process stage (i.e. from raw material to garment) with a homogeneous distribution of the components. It can be applied to different types of textile materials (e.g. staples, tops, yarns and fabrics) that contain wool, cashmere or yak fibres and their blends. The method is based on a preliminary identification of all fibres in the blend on the basis of their morphology, by light microscopy. The proteins are then extracted by a thiourea/urea/dithiothreitol (DTT) solution. An enzymatic digestion by trypsin of the protein extracted from the fibres is carried out. Analysis of the ... |
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