STN EN ISO 11063
| Name: | STN EN ISO 11063 |
| Validity: | Valid |
| Number of pages: | 24 |
| Language: |
EN
|
| Paper: | 14,10€ |
| Electronic version |
a) Only read (without ability to print and copy)
12,69€ b) Without ability to print, with ability to copy (printscreen) 14,10€ c) With ability to print and copy (printscreen) 18,33€ |
| Slovak title | Kvalita pôdy. Priama extrakcia pôdnej DNA (ISO 11063: 2020) |
| English title | Soil quality - Direct extraction of soil DNA (ISO 11063:2020) |
| Release Date: | 01. 03. 2021 |
| Date of withdrawal: | |
| ICS: | 13.080.30 |
| Sorting character/National clasification code | 46 5218 |
| Level of incorporation: | idt EN ISO 11063:2020, idt ISO 11063:2020 |
| Official Journal | 02/21 |
| Amendments | |
| Replaced by: | |
| Repleces: | STN EN ISO 11063:2013-06 (46 5218) |
| Note in Official Journal: | |
| Subject of the standard: | The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals. The direct extraction of DNA from soil samples provides unique insight into the ?- and ß-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments. |
| Preview: | Náhľad normy (PDF) |