STN P CEN/TS 17883
| Name: | STN P CEN/TS 17883 |
| Validity: | Valid |
| Number of pages: | 24 |
| Language: |
EN
|
| Paper: | 14,10€ |
| Electronic version |
a) Only read (without ability to print and copy)
12,69€ b) Without ability to print, with ability to copy (printscreen) 14,10€ c) With ability to print and copy (printscreen) 18,33€ |
| Slovak title | Environmentálna charakterizácia výluhov z odpadu a pôdy s použitím reprodukčnej a toxikologickej génovej expresie v Daphnia magna |
| English title | Environmental characterization of leachates from waste and soil using reproductive and toxicological gene expression in Daphnia magna |
| Release Date: | 01. 03. 2023 |
| Date of withdrawal: | |
| ICS: | 13.030.01, 13.060.70, 13.080.99 |
| Sorting character/National clasification code | 83 8284 |
| Level of incorporation: | idt CEN/TS 17883:2022 |
| Official Journal | 02/23 |
| Amendments | |
| Replaced by: | |
| Repleces: | |
| Note in Official Journal: | Táto predbežná slovenská technická norma je určená na overenie. Prípadné pripomienky pošlite do decembra 2024 Úradu pre normalizáciu, metrológiu a skúšobníctvo Slovenskej republiky. |
| Subject of the standard: | This document specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to quantify the abundance of specific mRNA molecules extracted from Daphnia magna. The method allows the identification of molecular responses to exposures for potentially toxic substances through the analysis of the abundance of specific mRNA molecules. In this document, the central genes involved in reproductive and toxic responses are included. NOTE The selection of genes can be adapted to specific exposure conditions, for example, exposure to known toxic substances, by adding genes known to respond to a specific insult. The present method allows for rapid, robust and sensitive detection of molecular responses and can be used to analyse the toxic effects of water leachates from soil and waste. |
| Preview: | Náhľad normy (PDF) |